Opportunities

Research Technician (part time)
Research Assistant (part time)
Cancer Systems Biology Postdoctoral Positions
Lab Animal Research Technician
Research Technician I

Lynn Hlatky's advice on careers in Systems Biology.

Research Technician I, Part-Time 20-30 hrs

Job Description:

Transgenic and knockout mouse work- breeding, maintaining and genotyping (for genotyping blood collection or tail snips) of WT controls and different transgenic and knockout mice. Performing animal surgeries for various murine (mouse and rat) and rabbit surgical models, implantation procedures on mice (e.g. VEGF pellets, in slow-release pumps, etc), hind limb ischemia surgery model, acute myocardial ischemia surgery model, induction of chronic heart failure model and corresponding readout techniques (LDPI, Echo, Hemodynamic, etc). Bone marrow transplantation (BMT) models including gamma irradiation of mice in preparation for BMT, isolation of BM cells from femur and tibia, gradient centrifugation and tail-vein or intracardiac injections of isolated BM cells for engraftment. Implantation of various mouse tumor cell lines into flanks of mice in the Martigel mix or tail-vein injections of tumor cell lines. To monitor this models will need to perform Laser Doppler Perfusion Analysis, Echocardiography, anesthesia, tumor measurements on live animals (calipers), data collection for all above mentioned analyses. Tissue collection, embedding into OCT preservative, or fixation in various fixatives. Process for various immunostainings. Use cryostat to cut frozen tissue and process for histo- or immunoflourescent stainings.
Cell and Molecular Biology work- FACS analysis of normal and tumor cells, FACS sorting of bone marrow-derived stem and progenitor cells. Gamma-irradiation of cells and live animals followed by collection of hearts, bone marrow and other internal organs. Western, Northern and Southern blots, qRT-PCR, transcriptional and micro-RNA profiling.
Microscopy- Morphometry with Light and Fluorescent microscopy, time-lapse live cell microscopy, confocal microscopy.
Space Radiation work- Participation in space radiation experiments at NASA Space Radiation Laboratory (NSRL) located in Brookhaven National Laboratories (BNL), Long Island, NY. This will include: preparation of animals and cells to be transported to BNL, travel and stay at BNL for 4-7 days at least 3 times a year. Perform radiation experiments, at NSRL, and work at the Biology Department at BNL with samples of cells and animal tissue post-irradiation.

Education/Training:

BA/BS degree from an accredited college and at least 2-3 years of research laboratory experience.

Apply:

Please send your application and CV to attention of Drs. David Goukassian and Xinhua Yan.

David A. Goukassian, MD, PhD and Xinhua Yan, MD
Associate Professor of Medicine,
Tufts University School of Medicine
Principal Investigator,
Center for Cardiovascular Research and
Center of Cancer Systems Biology,
St. Elizabeth's Medical Center
736 Cambridge Street, CBR-307
Boston, MA 02135
Phone: 617-789-3143
Emails: david.goukassian [at] tufts.eduand xinhua.yan [at] tufts.edu.

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Research Assistant, Part Time 20-30 hrs

Job Description:

Working under the direction of/and reporting to Senior Laboratory Technician and working and assisting all members of the laboratory including research technicians and post-doctoral fellows;
Any cell culture work related to a specific project which includes growing cells, various treatments of cells, transfection experiments, endothelial progenitor cell (EPC) isolation and culturing;
Protein extraction of cell and tissue samples;
SDS PAGE/Western blot analysis of protein samples;
DNA extraction of cell and tissue samples and PCR analysis of DNA samples (for example, genotyping mice, ordering primers and related PCR products etc.);
Plasmid DNA preparation and restriction digest;
Preparation of all buffers and solutions in connection with the above mentioned duties, for example, SDS PAGE running buffer for protein sample gels, agarose gel running buffer for DNA sample gels, etc;
RNA extraction of cell and tissue samples, reverse transcription and Real Time PCR;
Participation in the space radiation studies at NASA Space Radiation Laboratory at Brookhaven National Laboratory, Long Island, NY;
Ordering of any reagents and supplies needed for procedures described in paragraphs above;
Calling clinical engineering for maintenance and repair of common lab equipment.

Education/Training:

BA/BS degree from an accredited college, should be willing to be trained in new methodologies, prior research lab experience a plus.

Apply:

Please send your application and CV to attention of Drs. David Goukassian and Xinhua Yan.

David A. Goukassian, MD, PhD and Xinhua Yan, MD
Associate Professor of Medicine,
Tufts University School of Medicine
Principal Investigator,
Center for Cardiovascular Research and
Center of Cancer Systems Biology,
St. Elizabeth's Medical Center
736 Cambridge Street, CBR-307, Boston, MA 02135
Phone: 617-789-3143
Emails: david.goukassian [at] tufts.eduand xinhua.yan [at] tufts.edu.

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Cancer Systems Biology Postdoctoral Positions

The Center of Cancer Systems Biology at St. Elizabeth's Medical Center, Tufts University School of Medicine, seeks motivated individuals seasoned in mathematical analysis or radiation biology/physics who are interested in modeling aspects of cancer development and therapeutic response, including angiogenesis, inter-tissue interactions, gene network logic, DNA damage and matrix remodeling. They will join a team of well-established cancer biologists, molecular biologists, clinicians and mathematicians at the Center. Beyond the oncogenic events within tumor cells it is now appreciated that stromal/endothelial/tumor-cell interaction dynamics play a critical role in tumor advancement. Of particular importance is the establishment of biomathematical tumor models and simulation platforms upon which hypotheses related to tumor growth dynamics may be studied. Opportunity for in vitroand animal experimentation is available.

Two-year positions are available immediately, renewable upon performance. Opportunity exists for advancement to faculty level. Preferred candidates will be PhDs who are familiar with partial differential equation, numerical simulation and/or discrete or stochastic algorithmic approaches to modeling dynamical phenomena in biology. Background in the issues related to tumor development, tumor risk, clinical therapeutic control strategies, radiation response, radiation risk, digitized imaging or gene network modeling theory a plus.

Apply:

Please submit a letter of intent, curriculum vitaeand the names and addresses of two references to:

Dr. Philip J. Hahnfeldt
Center of Cancer Systems Biology
St. Elizabeth's Medical Center
Tufts University School of Medicine
736 Cambridge St., CBR-115
Boston, MA 02135-2997
Tel: 617-789-2998
Fax: 617-779-6572
Page: 617-362-6437
Email: Philip.Hahnfeldt [at] tufts.edu

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Lab Animal Research Technician

Job Description:

Occupational Summary: Responsible for maintaining detailed records and assist in procedures in an animal research facility.

Work Performed:

Work under the direction of/and report to Lab Animal Research Manager;
Perform necropsies, tissue collection and specimen photography;
Maintain breeding colonies and colony records;
Collect daily records of animal weights and other parameters;
Assist Lab Animal Research Manager;
Perform other related duties incidental to the work described herein.

The above statements describe the general nature and level of work being performed. This is not intended to be an exhaustive list of all responsibilities and duties required of personnel so classified.

Education/Training:

High School or College degree. LAT certification preferred.

Apply:

Please send your application and CV to:
Dr. Lynn Hlatky
Center of Cancer Systems Biology
St. Elizabeth's Medical Center
Tufts University School of Medicine
736 Cambridge St.
Boston, MA 02135-2997
Tel: 617-789-2997
Fax: 617-789-3111
Email: lynn.hlatky [at] tufts.edu

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Research Technician I

Job Description:

Under the direction of the Director performs experiments designed to study cancer. Responsible and dedicated individual capable of quality performance without constant supervision. Performs routine and standardized tasks including cell culture and laboratory animal studies of various laboratory animals. Responsible for maintaining the cell culture facility and experiments for the Center of Cancer Systems Biology. Responsibilities include recording experiments and results in a laboratory notebook, light laboratory maintenance duties, and training other staff.

Qualifications:

BA or BS from an accredited 4-year college or equivalent laboratory experience. Cell culture experience required. Experience with histology and laboratory animal a plus.

Apply:

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St. Elizabeth's Medical Center is an Affirmative Action/Equal Opportunity Employer

You may contact Lynn Hlatkyor Philip Hahnfeldtto inquire about future opportunities.