Figure 3. A murine protein-protein interaction (PPI) network for signaling proteins assessed from the regulation of gene expressions. For A) & B) the network contains 281 murine proteins linked by 551 unique interactions. Blue nodes represent proteins that are in the network but are not regulated by the ⁵⁶Fe irradiation perturbation, red nodes are determined to be up-regulated, and green nodes are down-regulated. A) PPI network showing regulations of tumors growing in young ⁵⁶Fe-irradiated mice as compared with those in young unirradiated mice. B) PPI network regulations in tumors growing in middle-age ⁵⁶Fe-irradiated mice compared with those in middle-aged unirradiated mice. For (C) – (F) pathway analysis was done with Ingenuity Pathway Analysis (IPA) software. Network depicted contains central nodes from AKT1 and FASN with direct (— solid lines) and indirect (- - dashed lines) relations to these molecules. Log₂ fold changes to the gene expression were used to obtain different shades of green for regulation levels for down-regulated genes, while different shades of red depict regulation levels for up-regulated genes. Grey genes exist in the network without a significant 2-fold change under the perturbation investigated. The darker the shade of green or red, the greater the fold change.

Reference: Age and space irradiation modulate tumor progression: implications for carcinogenesis risk. Beheshti A, Sachs RK, Peluso M, Rietman E, Hahnfeldt P, Hlatky L. Radiat Res. 2013 Feb;179(2):208-20. Epub 2013 Jan 4.]

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